{"id":3547,"date":"2021-09-09T11:23:13","date_gmt":"2021-09-09T11:23:13","guid":{"rendered":"https:\/\/www.biolyotech.com\/?page_id=3547"},"modified":"2023-08-03T09:43:58","modified_gmt":"2023-08-03T09:43:58","slug":"medium-development","status":"publish","type":"page","link":"https:\/\/www.biolyotech.com\/index.php\/live-bacterial-product-services\/medium-development\/","title":{"rendered":"Medium Development"},"content":{"rendered":"<div>\n<p>The basis of all LBP manufacture is having a medium that supports reproducible and robust growth of the organism of interest. Since developing a defined medium can take a long time, a commonly taken approach is to add many complex components to a medium so that the organism will grow to at least a minimum density required to generate clinical material. The trouble with this approach is that many complex materials such as partially digested plant or animal proteins show a considerable batch to batch variation in composition which affects the reproducibility of the fermentation. Additionally, a number of amino acids or other components present in complex raw materials have the potential to interfere with growth rather than support it.<\/p>\n<\/div>\n<div>\n<p>BioLyo\u2019s approach to medium development is to minimize the contribution of complex raw materials in the medium, and identifying which substrates are essential to an organisms&#8217; growth. Additionally, this approach is quite standardized and leads to practical results quickly. Using publicly available literature and metabolic databases has greatly facilitated medium development, i.e. after a couple of iterations in shake flasks or Hungate tubes the main substrates are identified and used to create a medium that supports robust growth in a medium with very limited complex materials. Usually this leads to a medium that supports growth to higher cell densities than the media described in literature, providing far better control of the fermentation.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The basis of all LBP manufacture is having a medium [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":3878,"parent":3544,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"side-navigation.php","meta":{"footnotes":""},"class_list":["post-3547","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/pages\/3547","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/comments?post=3547"}],"version-history":[{"count":6,"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/pages\/3547\/revisions"}],"predecessor-version":[{"id":4042,"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/pages\/3547\/revisions\/4042"}],"up":[{"embeddable":true,"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/pages\/3544"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/media\/3878"}],"wp:attachment":[{"href":"https:\/\/www.biolyotech.com\/index.php\/wp-json\/wp\/v2\/media?parent=3547"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}